Urease test: kuyini nokuthi kwenziwa kanjani

-Delile

• Ukuhlolwa kwenziwa kanjani
• Uwuqonda kanjani umphumela

Ukuhlolwa kwe-urease ukuhlolwa kwelabhorethri okusetshenziselwa ukukhomba amabhaktheriya ngokuthola umsebenzi we-enzyme okungenzeka ukuthi amabhaktheriya angenayo noma angenayo. I-Urease iyi-enzyme ebhekele ukwehlukaniswa kwe-urea ibe yi-ammonia ne-bicarbonate, okwandisa i-pH yendawo lapho ikhona, ikhetha ukwanda kwayo.

Lokhu kuhlolwa kusetshenziswa ikakhulu ekutholeni ukutheleleka nge I-Helicobacter pylori, noma H. pylori, obhekene nezinkinga eziningana, njenge-gastritis, esophagitis, duodenitis, ulcer nomdlavuza wesisu, ngenxa yalesi sizathu. Ngakho-ke, uma kukhona ukusola kokutheleleka ngo H. pylori, i-gastroenterologist ingenza isivivinyo se-urease ngesikhathi se-endoscopy. Uma kunjalo, ukwelashwa kuqalwa ngokushesha ngenhloso yokuvimbela lesi sifo ukuthi sikhule futhi sinciphise izimpawu zomuntu.

Ukuhlolwa kwenziwa kanjani

Lapho ukuhlolwa kwe-urease kwenziwa njengenqubo ejwayelekile yelabhorethri, akukho ukulungiselela okudingekayo ekuhlolweni. Kodwa-ke, uma kwenziwa ngesikhathi se-endoscopy, kubalulekile ukuthi umuntu alandele yonke imithetho yokuhlolwa, njengokugwema ukusebenzisa izidakamizwa zokulwa nokuzila ukudla okungenani amahora ayi-8.

Isivivinyo se-urease senziwa elabhoratri ngokuhlaziywa kwento eqoqiwe, ngokuhlukaniswa kwe-microorganism okwenziwayo kanye nokuhlolwa kokuhlonza kwamakhemikhali, phakathi kwabo ukuhlolwa kwe-urease. Ukwenza isivivinyo, i-microorganism ehlukanisiwe igonywe esikweni lesiko eliqukethe i-urea kanye ne-phenol red pH indicator. Ngemuva kwalokho, kuhlolwe ukuthi ngabe kukhona ushintsho kumbala wendawo noma cha, okukhombisa ubukhona nokungabikho kwamagciwane.

Endabeni yokuhlolwa kwe-urease ukuthola ukutheleleka nge H. pylori, ukuhlolwa kwenziwa ngesikhathi sokuhlolwa okuphezulu kwe-endoscopy, okuwukuhlolwa okuhlola impilo yomphimbo nesisu, ngaphandle kokubanga ubuhlungu noma ukungaphatheki kahle esigulini futhi umphumela ungahlolwa emizuzwini embalwa. Ngesikhathi sokuhlolwa, kususwa ucezu oluncane lodonga lwesisu lubekwe kuflaski equkethe i-urea nesikhombi se-pH. Uma ngemuva kwemizuzu embalwa okuphakathi kuguquka umbala, ukuhlolwa kuthiwe kune-urease positive, kuqinisekisa ukutheleleka ngo- H. pylori. Bona ukuthi yiziphi izimpawu ezingakhombisa ukutheleleka ngo- H. pylori.

Uwuqonda kanjani umphumela

Umphumela wokuhlolwa kwe-urease unikezwa kusuka ekushintsheni kombala kokuphakathi okwenziwa khona ukuhlolwa. Ngakho-ke, imiphumela ingaba:

• Oqondile, lapho ibhaktheriya ene-enzyme urease ikwazi ukwehlisa isithunzi i-urea, okwenza i-ammonia ne-bicarbonate, lokhu kusabela kutholakale ngokushintsha umbala wento ephakathi nendawo, eshintsha isephuzi iye kupinki / kubomvu.
• Okubi lapho kungekho shintsho kumbala wendawo, okukhombisa ukuthi ibhaktheriya ayinayo i-enzyme.

Kubalulekile ukuthi imiphumela ihunyushwe kungakapheli amahora angama-24 ukuze kungabi namathuba okuthola imiphumela engeyona eyamanga, okuyilawo okuthi ngenxa yokuguga kwalowo ophakathi, i-urea iqala ukwehliswa, engashintsha umbala.

Ngaphezu kokukhomba ukutheleleka nge I-Helicobacter pylori, ukuhlolwa kwe-urease kwenziwa ukukhomba amabhaktheriya amaningana, futhi ukuhlolwa nakho kune- I-Staphylococcus saprophyticus, I-Staphylococcus epidermidis, I-Proteus spp. futhi UKlebsiella pneumoniae, Ngokwesibonelo.