Ibala le-Gram: lenziwa kanjani nokuthi lenzelwe ini

-Delile

• Kwenziwa kanjani ibala le-Gram
• Yenzelwe ini

Ibala le-Gram, noma i-Gram nje, kuyindlela esheshayo nelula ehlose ukuhlukanisa amabhaktheriya ngokuya ngezimpawu zodonga lwamaseli abo ngemuva kokuchayeka odayi nezixazululo ezahlukahlukene.

Ngakho-ke, ngokusebenzisa i-Gram staining, kungenzeka ukuqinisekisa, ngaphezu kokuma kwamagciwane, umbala abawutholayo, futhi lo mphumela ubalulekile ekuchazeni amanye amasu okukhomba izinhlobo zebhaktheriya nokuthi udokotela akhombise ukwelashwa kokuvikela ngokusho kwezici ezibonwa nge-microscopically.

I-Gram staining ivame ukwenziwa njalo elabhorethri futhi iyingxenye yokuhlolwa kwe-bacterioscopy. Qonda ukuthi yini i- bacterioscopy nokuthi yenziwa kanjani.

Kwenziwa kanjani ibala le-Gram

I-Gram stain iyindlela esheshayo, esebenzayo futhi engabizi kakhulu yokuhlonza amabhaktheriya abhekene nokutheleleka, okuwusizo odokotela ukukhombisa ukwelashwa kokuvimbela ukutheleleka okungenzeka kwenzeke, ngoba izici ezithile zala maqembu amabhaktheriya ziyaziwa,

Ukufakwa kwe-Gram kwenziwa ngezinyathelo eziyisi-7 eziyinhloko, noma kunjalo umthetho olandelwayo ungahluka ngokuya nge-laboratory:

1. Beka amanye amakoloni wegciwane kusilayidi, engeza iconsi lamanzi ukwenza lula ukuhlanganiswa kwamakholoni;
2. Ake yome kancane, futhi i-blade ingadlula ngokushesha ilangabi ukuze ithande ukoma, kepha kubalulekile ukuthi unake izinga lokushisa, ngoba uma izinga lokushisa liphezulu kakhulu kungenzeka ukuthi kube noshintsho esakhiweni amabhaktheriya, angaphazamisa umphumela wokuhlolwa;
3. Lapho isilayidi somile, vala ngombala we-crystal violet bese usiyeka sisebenze cishe umzuzu owodwa;
4. Geza isilayidi ngomsele wamanzi agobhozayo bese umboza isilayidi nge-lugol, enenhloso yokulungisa udayi oluhlaza okwesibhakabhaka, bese usivumela sisebenze umzuzu owodwa. Zombili lezi zinhlobo zamagciwane ziyakwazi ukumunca okuyinkimbinkimbi okwenziwe udayi kanye ne-lugol, kube luhlaza okwesibhakabhaka;
5. Ngemuva kwalokho, geza isilayidi ngamanzi agobhozayo bese ufaka i-95% yotshwala, uyishiye isebenze imizuzwana engama-30. Utshwala bungumsebenzi wokuqeda ulwelwesi lwe-lipid olwakha amagciwane angenayo i-gram-negative futhi, ngaleyo ndlela, kususwe okuyinkimbinkimbi okwenziwe phakathi kwedayi ne-lugol, kukhishwe la magciwane. Kodwa-ke, esimweni samabhaktheriya ane-gram-positive, utshwala bunciphisa udonga lweseli lwamabhaktheriya ane-gram-positive, lubangele ukuthi izimbotshana zibambe futhi zenze zingangeneki;
6. Ngemuva kwalokho, kufanele igezwe futhi ngaphansi kwamanzi agobhozayo bese imboza isilayidi ngodayi wesibili, i-fuchsin noma i-safranin bese siyivumela ukuthi isebenze imizuzwana engama-30;
7. Ngemuva kwalokho, geza isilayidi ngaphansi kwamanzi agobhozayo bese uyivumela ukuthi yome ekamelweni lokushisa.

Lapho isilayidi somile, kungenzeka ukubeka ithonsi likawoyela wokucwilisa bese ubheka isilayidi ngaphansi kwesibonakhulu ngenhloso ye-100x, kungenzeka ukubheka ubukhona noma ukungabikho kwamagciwane, kanye nokuba khona kwemvubelo namaseli we-epithelial.

Yenzelwe ini

Ibala le-Gram linenhloso eyinhloko yokuhlukanisa amabhaktheriya ngokuya ngezimpawu zodonga lweseli kanye ne-morphology ejwayelekile. Ngakho-ke, ngokuya ngezici ezibonwe ngaphansi kwesibonakhulu, amabhaktheriya angahlukaniswa abe:

• Ama-bacterium ane-Gram, ezibonakala ngombala oluhlaza okwesibhakabhaka ngenxa yokuthi azishintshwanga umbala ngotshwala, ngoba zinodonga lweseli olujiyile futhi izimbotshana zazo ziyaqina lapho zivezwa ku-lugol;
• Amagciwane angenayo i-Gram, ekhonjiswa ngombala opinki / onsomi ngenxa yokuthi ziphahlwe utshwala futhi zingcoliswe yi-safranin noma i-fuchsin.

Ngemuva kokubuka ngamagciwane ngaphansi kwesibonakhulu, kungenzeka ukuthi ezinye izivivinyo zenziwe elabhoratri ukukhomba izinhlobo zebhaktheriya. Kodwa-ke, ngeGram nokuhlangana nezimpawu nezimpawu ezethulwe ngumuntu, udokotela angakhombisa ukwelashwa kokuvikela kuze kutholakale imiphumela yezivivinyo ezithile, ngoba ngale ndlela kungenzeka ukunciphisa izinga lokuphindaphindeka kwamagciwane futhi kuvimbele izinkinga.