Ukuhlolwa kwegazi kwe-ELISA

I-ELISA imele i-immunoassay exhumene ne-enzyme. Kuluhlolo lwaselabhoratri olusetshenziswa kakhulu ukuthola amasosha omzimba egazini. I-antibody yiprotheni ekhiqizwa amasosha omzimba womzimba lapho ethola izinto eziyingozi, ezibizwa ngama-antigen.

Kudingeka isampula yegazi. Isikhathi esiningi, igazi likhishwa emthanjeni ongaphakathi endololwaneni noma ngemuva kwesandla.

Isampuli ithunyelwa elabhorethri lapho i-antibody noma i-antigen eqondisiwe ixhunywe ku-enzyme ethile. Uma into eqondiwe ikusampula, isisombululo sokuhlola siguqula umbala ohlukile.

Akukho ukulungiselela okukhethekile okudingekayo.

Lapho inaliti ifakwa ukudonsa igazi, abanye abantu bezwa ubuhlungu obulinganiselayo. Abanye bazizwa beshaywa noma betinyelwa kuphela. Ngemuva kwalokho, kungahle kube nokushaywa okuthile noma ukulimazeka okuncane. Lokhu maduzane kuyaphela.

Lokhu kuhlolwa kuvame ukusetshenziselwa ukubona ukuthi ngabe uvezwe yini ngamagciwane noma ezinye izinto ezibanga ukutheleleka. Ibuye isetshenziselwe ukubhekela ukutheleleka kwamanje noma okwedlule.

Amanani ajwayelekile ancike ohlotsheni lwezinto ezikhonjwayo. Amanye amalabhoratri asebenzisa izilinganiso ezahlukahlukene noma avivinye amasampula ahlukile. Ububanzi bamanani ajwayelekile bungahluka kancane phakathi kwamalabhorethri ahlukene. Khuluma nomhlinzeki wakho wezokunakekelwa kwempilo mayelana nencazelo yemiphumela yakho ethile yokuhlolwa.

Amanani angajwayelekile ancike ohlotsheni lwezinto ezikhonjwayo. Kwabanye abantu, imiphumela emihle ingaba evamile.

Kunengozi encane ethintekayo ekuthatheni igazi lakho. Imithambo nemithambo iyehluka ngobukhulu ukusuka komunye umuntu iye komunye, futhi kusukela kolunye uhlangothi lomzimba kuye kolunye. Ukuthatha igazi kwabanye abantu kungaba nzima kakhulu ukwedlula kwabanye.

Ezinye izingozi ezihambisana nokudonswa igazi zincane, kepha zingafaka:

• Ukopha ngokweqile
• Ukuquleka noma uzizwe unekhanda elincane
• Ukubhoboza okuningi ukuthola imithambo
• I-hematoma (ukuqoqwa kwegazi ngaphansi kwesikhumba)
• Ukutheleleka (ingozi encane noma kunini lapho isikhumba siphukile)

I-immunoassay exhumene ne-enzyme; I-EIA

• Ukuhlolwa kwegazi

Aoyagi K, Ashihara Y, Kasahara Y. Immunoassay kanye ne-immunochemistry. Ku: McPherson RA, Pincus MR, abahleli. UHenry’s Clinical Diagnosis and Management by Laboratory Methods. Umhla ka-23. ISt Louis, MO: Elsevier; I-2017: isahluko 44.

UMurray PR. Umtholampilo wasemtholampilo kanye ne-microbiology Ku: Bennett JE, Dolin R, Blaser MJ, ama-eds. Mandell, Douglas, kanye neBennett's Principles and Practice of Infectious Diseases, uHlelo Olubuyekeziwe. Umhlaka 8. IPhiladelphia, PA: Elsevier Saunders; 2015: isahluko 16.