Ukuhlolwa kwesikhohlela okuqondile kwe-anti-fluorescent antibody (DFA)

Isikhohlela ngqo se-fluorescent antibody (DFA) ukuhlolwa kwelebhu okubheka izinto eziphilayo ezincane emfihlakalweni yamaphaphu.

Uzokhiqiza isampula lesikhohlela emaphashini akho ngokukhwehlela amafinyila kusuka ekujuleni kwamaphaphu akho. (I-Mucus ayifani namathe noma amathe aphuma emlonyeni.)

Isampuli ithunyelwa ebhodini. Lapho, kufakwa idayi ye-fluorescent kusampula. Uma kunezinto ezincane eziphilayo, ukukhanya okukhanyayo (i-fluorescence) kungabonakala kusampula yesikhwehlela kusetshenziswa imicroscope ekhethekile.

Uma ukukhwehlela kungakhiphi isikhwehlela, ukwelashwa kokuphefumula kunganikezwa ngaphambi kokuhlolwa ukuqala ukukhiqizwa kwesikhwehlela.

Akukho ukungaphatheki kahle ngalokhu kuhlolwa.

Udokotela wakho angalalela lokhu kuhlolwa uma unezimpawu zezifo ezithile zamaphaphu.

Ngokuvamile, akukho ukusabela kwe-antigen-antibody.

Imiphumela engavamile ingahle ibangelwe ukutheleleka okufana nalokhu:

• Isifo seLegionnaire
• I-pneumonia ngenxa yamagciwane athile

Azikho izingozi ngalokhu kuhlolwa.

Ukuhlolwa okuqondile kwe-immunofluorescence; I-antibody eqondile ye-fluorescent - isikhwehlela

UBanaei N, Deresinski SC, Pinsky BA. Ukuxilongwa kweMicrobiologic kokutheleleka kwamaphaphu. Ku: Broaddus VC, Mason RJ, Ernst JD, et al, abakwa-eds. Incwadi kaMurray neNadel Yemithi Yokuphefumula. Umhlaka 6. IPhiladelphia, PA: Elsevier Saunders; 2016: isahluko 17.

Patel R. Umtholampilo kanye nelabhorethri yezinto eziphilayo ezincane: ukuhleleka kokuhlolwa, ukuqoqwa kwezinhlobonhlobo, nokuchazwa komphumela. Ku: Bennett JE, Dolin R, Blaser MJ, ama-eds. Mandell, Douglas, kanye neBennett's Principles and Practice of Infectious Diseases. Umhlaka 9. IPhiladelphia, PA: Elsevier; 2020: isahluko 16.